Experimental Physiology Celebrating 100 years
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Quarterly Journal of Experimental Physiology and Cognate Medical Sciences 28.1 pp 77-86
© The Physiological Society 1938
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clark, A. J.
Right arrow Articles by Stedman, E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Clark, A. J.
Right arrow Articles by Stedman, E.

KINETICS OF CHOLINE ESTERASE

A. J. Clark 1, J. Raventós 1, E. Stedman 1, and Ellen Stedman 1

1 Departments of Pharmacology and Medical Chemistry, University of Edinburgh

1. The rate of hydrolysis of Ac.Ch. by esterase has been measured by chemical and by biological methods and the results obtained show a good agreement.

2. With high concentrations of Ac.Ch. (more than 0·01 per cent.) the amount hydrolysed per sec. is constant.

3. With low concentrations of Ac.Ch. (less than 0·001 per cent. or 5 x 10-5 molar) the amount of Ac.Ch. hydrolysed per sec. varies as (conc. Ac.Ch.)0·83.

4. The amount of Ac.Ch. hydrolysed per sec. is approximately proportional to the esterase concentration.

5. These results indicate that with the undiluted stock esterase solution the time for half hydrolysis of low concentrations of Ac.Ch. (1 in 10 million) is about 0·2 sec.

The expenses of this research were defrayed by a grant from the Moray Fund of the University of Edinburgh, for which the authors desire to tender their thanks.

Submitted on April 27, 1938






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1938 by the The Physiological Society.