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Quarterly Journal of Experimental Physiology and Cognate Medical Sciences 41.2 pp 124-130
© The Physiological Society 1956
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ON THE FIXATION OF MUCIN AND THE PREPARATION OF AUTORADIOGRAPHS

N. G. Heatley 1, D. W. Jerrome 1, M. A. Jennings 1, and H. W. Florey 1

1 The Sir William Dunn School of Pathology, Oxford

1. Phosphomolybdic acid, a mucin precipitant, is a useful ingredient in a histological fixative designed for the preservation of intracellular mucin. By its use the mucin within goblet cells and some other mucous cells can be preserved in the form of fine, compact granules, with little distortion of the theca or other cell structures.

2. This effect is enhanced by embedding in a modified ester wax mixture, which apparently penetrates and supports the tissue better than paraffin wax.

3. Using sections prepared in this way, autoradiographs have been obtained in which the sites of radioactivity can be related with some degree of accuracy to the mucin within each cell.

Submitted on December 10, 1955






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Copyright © 1956 by the The Physiological Society.