AUTORADIOGRAPHIC OBSERVATIONS OF MANGANESE IN ADULT AND EMBRYO MICE

¹ Radioisotope Group, Laboratory of Nuclear Science, Faculty of Science, Osaka University, Osaka, Japan; Department of Nuclear Science, College of Science, University of Kyoto, Kyoto, Japan
² Radioisotope Group, Laboratory of Nuclear Science, Faculty of Science, Osaka University, Osaka, Japan; Department of Biology, Nara Medical University, Kashihara, Nara, Japan

In connection with the previous work, autoradiographic studies on the distribution of radiomanganese in the mouse tissues were carried out on the adult and the embryo.

Results from the adult are summarized as follows. In the kidney, ⁵²Mn is accumulated more abundantly in the cortex than in the medulla. On precise examinations, ⁵²Mn is detected remarkably within the epithelial cells of renal tubules but scarcely in the lumen. In the intestinal villi, ⁵²Mn is found conspicuously in the epithelium and in the mucus cohering to its free surface, which fact may offer a reasonable ground for manganese excretion through intestinal epithelium. ⁵²Mn is retained in the exocrine portion of the pancreas but not in the islets of Langerhans, and in the liver it is uniformly distributed over the tissues except the lumens of blood vessels. ⁵²Mn-retention in the blood is extremely small.

In the early embryos, ⁵²Mn-concentration is less than in the placental tissues. In the late embryos, ⁵²Mn-accumulation in the bone proceeds in parallel with the progress of ossification and ⁵²Mn-concentration in the kidney is less than in the liver, conversely in the case of the adult.

From the present autoradiographic observations, the physiological meaning of manganese found in tissues has been discussed with reference to the previous work.

Note:

This work was supported in part by a grant for Scientific Research from the Education Ministry of Japan.

We should like to acknowledge our great indebtedness to those who helped us during the progress of this work; Dr. T. Wakatsuki, Dr. S. Yamabe, Dr. K. Otozai and Dr. Y. Yoshizawa (Cyclotron Group and Radioisotope Group, LNS, FS, Osaka University) for production and separation of radiomanganese; Dr. I. Honjo (Department of Biology, FS, Osaka University) and Dr. H. Kitamura (Department of Pathology, Nara Medical University) for helpful suggestions and criticism from the view-point of a physiologist and a pathologist, respectively. We are also indebted to the Biological Laboratory of Osaka Women's University for microphotography. We wish to express our special gratitude to Dr. S. Kume (Department of Radiochemistry, FS, Osaka University) who gave us cordial guidance and valuable suggestions in the chemical procedures, and also to Dr. R. Hara, Mrs. K. Koshida and Miss K. Yashiki who cooperated with us in many ways throughout this work.

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