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Quarterly Journal of Experimental Physiology and Cognate Medical Sciences 48.4 pp 388-401
© The Physiological Society 1963
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ARTERIAL AND PORTAL VENOUS BLOOD FOR ESTIMATING MINERAL ABSORPTION IN THE CONSCIOUS SHEEP

E. W. Moodie 1 and A. I. T. Walker 1

1 Royal (Dick) School of Veterinary Studies, Edinburgh

A method is described for estimating the absorption of nutrients in conscious sheep by comparing the composition of arterial and portal venous bloods. One hundred and seventeen pairs of samples were obtained from sixteen sheep (seven castrated male and nine female) and analyzed for hæmoglobin, plasma calcium, magnesium, inorganic phosphate, citric acid and whole-blood chloride contents. Changes in the volume of the blood passing through the splanchnic area were estimated by comparing the hæmoglobin concentrations in arterial and portal blood samples.

The veno-arterial differences in the concentration of each constituent before and after correction for changes in the volume of the blood are presented and the necessity for making this correction is discussed. There is evidence of differences in the rate of absorption of calcium and chloride between male and female sheep and among individual female sheep, and also of differences in the absorption of calcium and chloride compared with magnesium and inorganic phosphate.

Note:

This work was largely financed by grants from the Agricultural Research Council. We are indebted to Miss Marit Mosvall for technical assistance and to Professor A. Robertson for his interest and encouragement.

Submitted on April 27, 1963






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Copyright © 1963 by the The Physiological Society.