THE EFFECTS OF VASOPRESSIN, THEOPHYLLINE AND CYCLIC 3'-5'-ADENOSINE MONOPHOSPHATE (CYCLIC AMP) ON SODIUM TRANSPORT ACROSS THE FROG SKIN

¹ Department of Pharmacology and Therapeutics, University of Sheffield

The effects of vasopressin, theophylline and cyclic AMP on sodium transport across isolated frog skin have been studied using ²⁴Na for the measurement of sodium fluxes. Both vasopressin and theophylline produced consistent increases in short-circuit current, sodium influx and efflux, with a reduction in partial resistance of the skin. The same results were obtained with cyclic AMP only when it was combined with small (loses of theophylline which were by themselves ineffective. The similarity of effect of these three compounds on sodium transport mechanisms strengthens the hypothesis that the physiological activity of vasopressin may be mediated by cyclic AMP.

Note:

We wish to thank Professor G. M. Wilson for advice and criticism and Mr. D. Gow and Mr. M. Wright for technical help. W.I.B. holds the J. G. Graves Medical Research Fellowship in the University of Sheffield. Both the Medical Research Council and the United Sheffield Hospitals Endowment Research Fund have provided financial support for this work.

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