Experimental Physiology
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Quarterly Journal of Experimental Physiology 71.3 pp 467-473
© The Physiological Society 1986
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FREE INTRACELLULAR MAGNESIUM CONCENTRATION IN FERRET VENTRICULAR MUSCLE MEASURED WITH ION SELECTIVE MICRO-ELECTRODES

L. A. Blatter 1 and John A. S. McGuigan 1

1 Department of Physiology, University of Berne, Buehlplatz 5, 3012 Berne, Switzerland

The free Mg concentration ([Mg]i) was measured in ferret ventricular muscle using single-barrelled Mg-sensitive micro-electrodes. The electrodes were calibrated in solutions containing the [K]i and [Na]i measured in this tissue. In thirteen measurements from seven experiments conforming to strict criteria for calibration and penetration the estimated mean [Mg]i was 0·4 mmol/l. This is to be regarded as an upper limit since it was estimated by linear extrapolation of the calibration curve.

Submitted on August 2, 1985




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