Experimental Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Quarterly Journal of Experimental Physiology 71.4 pp 689-698
© The Physiological Society 1986
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hay, W. W.
Right arrow Articles by Meznarich, H. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hay, W. W., Jr
Right arrow Articles by Meznarich, H. K.

THE EFFECT OF HYPERINSULINAEMIA ON GLUCOSE UTILIZATION AND OXIDATION AND ON OXYGEN CONSUMPTION IN THE FETAL LAMB

William W. Hay Jr 1 and Huei Kang Meznarich 1

1 The Division of Perinatal Medicine, Departments of Pediatrics and Physiology, University of Colorado School of Medicine, Denver, CO, U.S.A.

In order to measure the effect of hyperinsulinaemia on fetal glucose metabolism and oxygen consumption, we applied the glucose-clamp technique to experiments in fifteen late-gestation, unstressed, chronically catheterized fetal lambs. In a control period, and immediately thereafter following 2 h of hyperinsulinaemia, we measured fetal glucose utilization and oxidation rates (radioactive tracer methodology) and net fetal uptake rates of exogenous glucose and oxygen uptake rates (Fick principle). During the period of hyperinsulinaemia, fetal glucose concentration was maintained at the average control period value by a variable rate of glucose infusion into the fetus in response to serial 10 min measurements of fetal arterial blood glucose concentration. Hyperinsulinaemia in the fetus (12·12 ± 1·92 ng.ml-1 (mean ± S.E.M.) arterial plasma) resulted in a 13% increase in net fetal oxygen uptake rate (0·310 ± 0·011 to 0·349 ± 0·012 mmol.min-1.kg-1), a 106% increase of fetal glucose uptake rate (4·54 ± 0·43 to 9·35 ± 0·50 mg.min-1.kg-1) and an 83% increase of fetal glucose utilization rate (4·94 ± 0·43 to 9·05 ± 0·83 mg.min-1.kg-1). Fetal glucose uptake and utilization rates were not different from each other during the control and the hyperinsulinaemia periods. The fraction of glucose that was oxidized (0·58 ± 0·05, control; 0·54 ± 0·05, hyperinsulinaemia) did not change significantly; thus the glucose oxidation rate, the rate of entry of glucose into non-oxidative pathways, and the amount of oxygen used for glucose oxidation all increased in proportion to glucose utilization. These results suggest that insulin promotes the entry of glucose into fetal tissues, thereby increasing fetal glucose utilization and oxidation rates and substituting glucose oxidation for that of other substrates. The insulin-enhanced glucose utilization rate also increases slightly fetal metabolic rate.

Submitted on October 23, 1985




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. K. Philp, B. S. Muhlhausler, A. Janovska, G. A. Wittert, J. A. Duffield, and I. C. McMillen
Maternal overnutrition suppresses the phosphorylation of 5'-AMP-activated protein kinase in liver, but not skeletal muscle, in the fetal and neonatal sheep
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2008; 295(6): R1982 - R1990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. D. Brown and W. W. Hay Jr.
Effect of hyperinsulinemia on amino acid utilization and oxidation independent of glucose metabolism in the ovine fetus
Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1333 - E1340.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. Gray, B. S Stonestreet, S. Thamotharan, G. B Sadowska, M. Daood, J. Watchko, and S. U Devaskar
Skeletal muscle glucose transporter protein responses to antenatal glucocorticoids in the ovine fetus.
J. Endocrinol., May 1, 2006; 189(2): 219 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. S. Anderson, M. Thamotharan, D. Kao, S. U. Devaskar, L. Qiao, J. E. Friedman, and W. W. Hay Jr
Effects of acute hyperinsulinemia on insulin signal transduction and glucose transporters in ovine fetal skeletal muscle
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R473 - R481.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. U. Devaskar, R. Anthony, and W. Hay Jr.
Ontogeny and insulin regulation of fetal ovine white adipose tissue leptin expression
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2002; 282(2): R431 - R438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. S. Anderson, J. He, J. Flowers-Ziegler, S. U. Devaskar, and W. W. Hay Jr.
Effects of selective hyperglycemia and hyperinsulinemia on glucose transporters in fetal ovine skeletal muscle
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1256 - R1263.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. A. Liechty, D. W. Boyle, H. Moorehead, W.-H. Lee, X.-L. Yang, and S. C. Denne
Glucose and amino acid kinetic response to graded infusion of rhIGF-I in the late gestation ovine fetus
Am J Physiol Endocrinol Metab, September 1, 1999; 277(3): E537 - E543.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
B. S. Stonestreet, L. D. Boyle, A. Papparella, and D. J. Berard
Circulatory and Metabolic Effects of {alpha}-Adrenergic Blockade in the Hyperinsulinemic Ovine Fetus
Reproductive Sciences, September 1, 1996; 3(5): 241 - 249.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the The Physiological Society.