Experimental Physiology
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Quarterly Journal of Experimental Physiology 73.6 pp 995-1000
© The Physiological Society 1988
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OPTICAL MEASUREMENTS OF INTRACELLULAR pH IN INTACT ISOLATED MUSCLE FIBRES AND MUSCLE GROWTH CONES IN CULTURE

J. I. Gillespie 1 and R. R. Ribchester 2

1 Department of Physiological Sciences, University of Newcastle, Newcastle upon Tyne NE2 7RU
2 Department of Physiology, University Medical School, Teviot Place, Edinburgh EH8 9AG

Intracellular pH (pHi) has been measured in intact muscle fibres isolated from the mouse flexor digitorum brevis muscle, using the fluorescence of dicyanohydroquinone as an indicator. The pHi in fibres cultured from 1 to 14 days ranged from 7·0 to 8·1. With time in culture, isolated fibres developed growth cone-like extensions from their ends. These ‘myocones’ were significantly more alkaline by 0·1-0·2 pH units than the parent fibre. Cultured fibres regulated pHi in response to acid loading with an NH4Cl pulse with recovery rate constants of up to 0·8 pH units/min.

Submitted on April 25, 1988
Accepted on May 23, 1988






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Copyright © 1988 by the The Physiological Society.