Experimental Physiology
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Quarterly Journal of Experimental Physiology 74.4 pp 541-544
© The Physiological Society 1989
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DYE INJECTION CONFIRMS THE ELECTROPHYSIOLOGICAL IDENTIFICATION OF REGENERATING MUSCLE FIBRES IN THE RAT

B. D. Grubb 1 and J. B. Harris 2

1 Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh EH9 1QH
2 Muscular Dystrophy Group Research Laboratories, Newcastle General Hospital, Westgate Road, Newcastle Upon Tyne NE4 6BE

Microelectrodes were inserted at random into superficial fibres in rat regenerating soleus muscles. The fibres from which recordings of electrical properties were made were marked using the dye Lucifer Yellow CH and then processed for histological studies. It was shown that the microelectrodes had sampled small regenerating muscle fibres, and that random penetration does not select for any undamaged fibres that may be present.

Submitted on March 17, 1989
Accepted on March 31, 1989






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Copyright © 1989 by the The Physiological Society.