Experimental Physiology
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Quarterly Journal of Experimental Physiology 74.4 pp 553-556
© The Physiological Society 1989
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RESISTANCE TO ACID OF CANINE KIDNEY (MDCK) AND HUMAN COLONIC (T84) AND ILEO-CAECAL (HCT-8) ADENO-CARCINOMA EPITHELIAL CELL MONOLAYERS IN VITRO

A. B. Chan 1, C. N. Allen 1, N. L. Simmons 1, B. H. Hirst 1, and M. E. Parsons 2

1 Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, Newcastle upon Tyne NE2 4HH
2 Department of Gastrointestinal Pharmacology, Smith Kline & French Research Ltd, The Frythe, Welwyn, Herts AL6 9AR

Monolayers of canine kidney (MDCK) and human lower intestinal (T84 and HCT-8) cell lines generated significant transepithelial electrical resistance (700-5000 OHgr cm2). Electrical integrity was maintained upon acidification of the apical and/or basolateral surfaces to pH 3·0, and this was associated with increased transepithelial electrical resistance, and generation of a potential difference at pH lang 4·5. These results indicate that resistance to acid is a general phenomenon of epithelial layers, and that monolayers of epithelial cells, including those of human origin, are a homogeneous and simple model for studying epithelial barrier function in vitro.

Submitted on April 3, 1989
Accepted on April 7, 1989






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Copyright © 1989 by the The Physiological Society.