Experimental Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Experimental Physiology 75.2 pp 199-209
© The Physiological Society 1990
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mistry, D.
Right arrow Articles by Cottrell, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mistry, D.
Right arrow Articles by Cottrell, G.
Experimental Physiology, Vol 75, Issue 2, 199-209
Copyright © 1990 by The Physiological Society

Article

Actions of steroids and bemegride on the GABAA receptor of mouse spinal neurones in culture

DK Mistry and GA Cottrell

The effects of a synthetic and an endogenous steroid were studied on the GABAA receptors of isolated mouse spinal neurones, maintained in culture. Low doses of alphaxalone reversibly increased GABA-evoked whole-cell currents. Alphaxalone at higher doses (10-50 microM), when pressure ejected onto spinal neurones, also directly evoked a membrane chloride current. Such currents were reversibly suppressed by bicuculline (a GABAA antagonist) and enhanced by phenobarbitone. 5 beta-Pregnan-3 alpha-ol-20-one, a progesterone metabolite, dose-dependently potentiated the amplitude of GABA-evoked whole-cell currents. The mechanism of potentiation was examined at the single-channel level using outside-out patches from spinal neurones. The main action of the steroid on the GABAA receptor appears to be similar to that found for barbiturates, in that they prolonged GABA-activated bursts of channel openings. Bemegride had an antagonistic action on the GABAA receptor, suppressing both GABA- and pentobarbitone-evoked whole-cell currents to similar extents.


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
M. T. Bianchi and R. L. Macdonald
Neurosteroids Shift Partial Agonist Activation of GABAA Receptor Channels from Low- to High-Efficacy Gating Patterns
J. Neurosci., November 26, 2003; 23(34): 10934 - 10943.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
L. Winter, R. Nadeson, A. P. Tucker, and C. S. Goodchild
Antinociceptive Properties of Neurosteroids: A Comparison of Alphadolone and Alphaxalone in Potentiation of Opioid Antinociception
Anesth. Analg., September 1, 2003; 97(3): 798 - 805.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. M. Wohlfarth, M. T. Bianchi, and R. L. Macdonald
Enhanced Neurosteroid Potentiation of Ternary GABAA Receptors Containing the delta Subunit
J. Neurosci., March 1, 2002; 22(5): 1541 - 1549.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Y. Valeyev, J. C. Hackman, A. M. Holohean, P. M. Wood, J. L. Katz, and R. A. Davidoff
Alphaxalone Activates a Cl- Conductance Independent of GABAA Receptors in Cultured Embryonic Human Dorsal Root Ganglion Neurons
J Neurophysiol, July 1, 1999; 82(1): 10 - 15.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. J. Zhu and S. Vicini
Neurosteroid Prolongs GABAA Channel Deactivation by Altering Kinetics of Desensitized States
J. Neurosci., June 1, 1997; 17(11): 4022 - 4031.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the The Physiological Society.