Experimental Physiology
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Experimental Physiology 76.5 pp 807-810
© The Physiological Society 1991
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Experimental Physiology, Vol 76, Issue 5, 807-810
Copyright © 1991 by The Physiological Society


Article

Primary cultures of guinea-pig gall-bladder epithelial cells suitable for patch-clamp studies

JA O'Brien, PS James, and M Torre

Cells derived from explants of guinea-pig gall-bladder maintained in culture for up to 14 days, exhibit a network of cytoskeletal filaments that are labelled by an anti-cytokeratin antibody, but not by an anti-desmin antibody, suggesting that they are of epithelial origin. These cells proved suitable for patch-clamp studies and by using this method channels selective for K+ and Cl- were observed.


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Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. J. Gunter-Smith, O. Abdulkadir, L. Hammonds-Odie, M. Scanlon, and R. Terrell
A primary culture of guinea pig gallbladder epithelial cells that is responsive to secretagogues
Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G866 - G874.
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