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Experimental Physiology 77.6 pp 929-932
© The Physiological Society 1992
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Experimental Physiology, Vol 77, Issue 6, 929-932
Copyright © 1992 by The Physiological Society

Article

Confocal analysis of fluorescent bead uptake by mouse Peyer's patch follicle-associated M cells

C Porta, PS James, AD Phillips, TC Savidge, MW Smith, and D Cremaschi

Latex beads coated with secretory immunoglobulin (IgA) instilled into mouse intestinal loops are taken up by M cells present in Peyer's patch follicle-associated epithelial tissue. Bead adsorption and uptake is greater at the edge compared with the apex of follicle domes. Coating beads with bovine serum albumin (BSA) causes a fourfold reduction in adsorption and a twentyfold reduction in uptake. Results demonstrate selectivity between adsorption and uptake and between the ability of different proteins to facilitate uptake.


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