Experimental Physiology
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Experimental Physiology 78.1 pp 109-112
© The Physiological Society 1993
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Experimental Physiology, Vol 78, Issue 1, 109-112
Copyright © 1993 by The Physiological Society

Article

Enterocytes on rat jejunal villi but not in the crypts posses m3 mRNA for the M3 muscarinic receptor localized by in situ hybridization

SA Przyborski and RJ Levin

Localization of m3 mRNA, for the expression of the M3 muscarinic receptor, along the crypt-villus axis, was undertaken in rat jejunum by in situ hybridization. While enterocytes on the lower two-thirds of the villi showed the presence of m3 mRNA it was absent in the crypt enterocytes. This indicates that the final locus of muscarinically activated jejunal secretion is mediated by the M3 receptor on the enterocytes of the villi and not via the crypt cells.


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