Expression of c-fos protein was examined in the brains of sheep (n = 2 per treatment) subjected to physical stress in a transport simulator, the psychological stress of isolation, insulin-induced hypoglycaemia or intracerebroventricular (I.C.V.) injection of ovine corticotrophin-releasing hormone (CRH). As control procedures, two sheep were given I.C.V. injections of saline and one animal received no treatment. Fos expression was induced in the hypothalamic paraventricular nucleus by transport simulation, isolation and hypoglycaemia, and also in the hypothalamic supraoptic nucleus by hypoglycaemia. Labelled nuclei were also seen in the brainstem in the region of the area postrema following transport simulation, isolation and insulin treatments. These results indicate that c-fos expression in the ovine CNS is a useful marker for the identification of brain regions activated by stressors.

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