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Department of Pharmacology, Sahlgrenska Academy at Göteborg University, Medicinaregatan 15 D, Göteborg 413 90, Sweden

In anaesthetized female rats, the ß-adrenoceptor agonist isoprenaline was intravenously infused (20 µg kg^–1 min^–1) for 30 min or the ascending cervical sympathetic nerve trunk was intermittently stimulated (50 Hz, 1 s every tenth second) on one side for 30 min. The incorporation of [³H]leucine into trichloroacetic acid (TCA)-insoluble material was used as an index of protein synthesis. In response to isoprenaline, the [³H]leucine incorporation increased by 79% in the parotid glands and by 82% in the submandibular glands. The neuronal type NO-synthase inhibitor N-PLA, reduced (P < 0.001) this response to 26% and 20%, respectively. Sympathetic stimulation under -adrenoceptor blockade increased the [³H]leucine incorporation by 192% in the parotid glands and by 35% in the submandibular glands. N-PLA reduced the corresponding percentage figures to 86% (P < 0.01) and 8% (P < 0.05). When tested in the parotid glands, the non-selective NO-synthase inhibitor L-NAME reduced (P < 0.01) the nerve-evoked response to 91%. The increase in [³H]leucine incorporation in response to sympathetic stimulation under ß-adrenoceptor blockade was not affected by N-PLA in the parotid (139%versus 144%) and submandibular glands (39%versus 34%). In non-stimulated glands, the [³H]leucine incorporation was not influenced by the NO-synthase inhibitors. In conclusion, ß-adrenoceptor mediated salivary gland protein synthesis is largely dependent on NO generation by neuronal type NO-synthase, most likely of parenchymal origin.

(Received 29 August 2003; accepted after revision 8 January 2003)
Corresponding author J. Ekström: Department of Pharmacology, Sahlgrenska Academy at Göteborg University, Medicinaregatan 15 D, Göteborg 413 90, Sweden. E-mail: jorgen.ekstrom{at}pharm.gu.se