| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Research Group for Paediatrics and Nephrology, Hungarian Academy of Sciences, Budapest, Bókay u 53, Hungary, H-10832 1st Department of Paediatrics, Semmelweis University, Budapest, Bókay u 53, Hungary, H-10833 Department of General Zoology, Eötvös University of Sciences, Budapest, Pázmány Péter sétány 12, Hungary, H-11174 2nd Department of Pathology, Semmelweis University, Budapest, Üllöi u 93, Hungary, H-1095 Department of Pulmonology, Semmelweis University, Budapest, Diósárok u 1/C, Hungary, H-1125
Vascular endothelial growth factor (VEGF) is a potent regulator of angiogenesis and vascular protection. Synthesis of VEGF is induced by hypoxia and different cytokines including interleukin-6 (IL-6) and interleukin-1ß (IL-1ß). However, post-ischaemic alterations of this growth factor in the kidney are incompletely known. To determine VEGF synthesis in renal ischaemia/reperfusion (I/R) injury unilateral warm ischaemia was induced by cross-clamping the left renal pedicle for 55 min followed by 2 and 24 h of reperfusion (T2 and T24 kidneys; n= 6 in each group). Sham-operated, non-clamped animals served as controls (n= 6). Renal VEGF, IL-6 and IL-1ß mRNA expression were determined by reverse transcription-polymerase chain reaction (RT-PCR). VEGF protein level and distribution were determined by Western blot and immunohistochemical analysis. Immunohistochemistry revealed prominent VEGF staining in the outer medulla of control, T2 and T24 kidneys. VEGF immunoreactivity accumulated at the basolateral area of tubular epithelial cells in T2 kidneys, while it was diffuse in control and T24 kidneys. VEGF protein levels were increased 2- to 3-fold in T2 and T24 kidneys (both P < 0.01 versus controls), while VEGF mRNA expression remained unchanged. IL-6 mRNA expression was increased (P < 0.01 versus controls) in T2 kidneys, while IL-1ß mRNA expression remained unchanged. Increased VEGF protein levels but not mRNA expression suggests that during renal I/R injury VEGF synthesis in kidneys – unlike in other organs – is primarily regulated at a post-transcriptional level. As IL-6 mRNA expression increased simultaneously with VEGF protein levels, the post-ischaemic regulation of IL-6 and VEGF synthesis might be interrelated in rat kidney.
(Received 26 February 2004;
accepted after revision 26 April 2004; first published online 6 May 2004)
Corresponding author A. Vannay: 1st Department of Paediatrics, Semmelweis University, 1083 Budapest, Bókay J. u. 53-54, Hungary. Email: vannay{at}gyer1.sote.hu
This article has been cited by other articles:
|
|
 |
|
  D. P. Basile, K. Fredrich, B. Chelladurai, E. C. Leonard, and A. R. Parrish Renal ischemia reperfusion inhibits VEGF expression and induces ADAMTS-1, a novel VEGF inhibitor Am J Physiol Renal Physiol, April 1, 2008; 294(4): F928 - F936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horbelt, S.-Y. Lee, H. E. Mang, N. L. Knipe, Y. Sado, A. Kribben, and T. A. Sutton Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury Am J Physiol Renal Physiol, September 1, 2007; 293(3): F688 - F695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Dong, Q-X Wang, C-Y Zhou, X-F Ma, and Y-C Zhang Activation of the STAT1 signalling pathway in lupus nephritis in MRL/lpr mice Lupus, February 1, 2007; 16(2): 101 - 109. [Abstract] [PDF]
|
|
|
|
 |
|
 E. B. Rankin, J. E. Tomaszewski, and V. H. Haase Renal cyst development in mice with conditional inactivation of the von hippel-lindau tumor suppressor. Cancer Res., March 1, 2006; 66(5): 2576 - 2583. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
