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This Article Full Text Full Text (PDF) All Versions of this Article: 89/5/559    most recent expphysiol.2004.027409v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kraniou, G. N. Articles by Hargreaves, M. Search for Related Content PubMed PubMed Citation Articles by Kraniou, G. N. Articles by Hargreaves, M. Related Collections Human, Environmental & Exercise

Centre for Physical Activity and Nutrition, School of Exercise & Nutrition Sciences, Deakin University, Burwood, 3125, Australia

Six untrained, male subjects (23 ± 1 years old, 84 ± 5 kg, _O2peak= 3.7 ± 0.8 l min^–1) exercised for 60 min at 75 ± 1%_O2peak on 7 consecutive days. Muscle samples were obtained before the start of cycle exercise training and 24 h after the first and seventh exercise sessions and analysed for citrate synthase activity, glycogen and glucose transporter 4 (GLUT-4) mRNA and protein expression. Exercise training increased (P < 0.05) citrate synthase by 20% and muscle glycogen concentration by 40%. GLUT-4 mRNA levels 24 h after the first and seventh exercise sessions were similar to those measured before the start of exercise training. In contrast, GLUT-4 protein expression was increased after 7 days of exercise training (12.4 ± 1.5 versus 3.4 ± 1.0 arbitray units (a.u.), P < 0.05) and although it tended to be higher 24 h after the first exercise session (6.0 ± 3.0 versus 3.4 ± 1.0 a.u.), this was not significantly different (P= 0.09). These results support the suggestion that the adaptive increase in skeletal muscle GLUT-4 protein expression with short-term exercise training arises from the repeated, transient increases in GLUT-gene transcription following each exercise bout leading to a gradual accumulation of GLUT-4 protein, despite GLUT-4 mRNA returning to basal levels between exercise stimuli.

(Received 8 February 2004; accepted after revision 2 June 2004; first published online 7 June 2004)
Corresponding author M. Hargreaves: Centre for Physical Activity and Nutrition, School of Exercise & Nutrition Sciences, Deakin University, Burwood, 3125, Australia. Email: mharg{at}deakin.edu.au

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