HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 89/5/617    most recent expphysiol.2004.027763v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stary, C. M. Articles by Hogan, M. C. Search for Related Content PubMed PubMed Citation Articles by Stary, C. M. Articles by Hogan, M. C. Related Collections Muscle

Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0623, USA

The purpose of the present study was to compare the individual fatigue characteristics of isolated single skeletal muscle fibres with their mitochondrial volume density (MVD), using direct histological morphometry. Single muscle fibres (n= 14) were microdissected from lumbrical muscle of adult female Xenopus laevis, and force was measured while fibres were stimulated (tetanic contractions of 200 ms trains with 70 Hz stimuli at 9 V) at progressively increasing frequencies (2 min each at 0.25, 0.33, 0.5 and 1 contractions s^–1) until fatigue (<50% initial maximal force) had been established. Following the end of the fatigue protocol, MVD was determined by electron microscopy. Time to fatigue varied among the individual fibres from 3.3 to 10 min. MVD of individual fibres ranged from 3.0 to 9.2% and was positively correlated (r= 0.93) with time to fatigue of corresponding fibres. These results, using direct histological measurements of MVD: (1) support on a single cell basis the notion that oxidative capacity is a major determinant of muscle fatigue resistance; and (2) show that the fatigue profile of a single cell can be used to predict oxidative capacity.

(Received 1 April 2004; accepted after revision 2 July 2004; first published online 15 July 2004)
Corresponding author M. C. Hogan: Department of Medicine, 0623-A, University of California, San Diego, La Jolla, CA 92093-0623, USA. Email: mchogan{at}ucsd.edu

This article has been cited by other articles:

				B. Walsh, C. M. Stary, R. A. Howlett, K. M. Kelley, and M. C. Hogan Glycolytic activation at the onset of contractions in isolated Xenopus laevis single myofibres Exp Physiol, September 1, 2008; 93(9): 1076 - 1084. [Abstract] [Full Text] [PDF]

				E. E. R. Philipp, M. Schmidt, C. Gsottbauer, A. M. Sanger, and D. Abele Size- and age-dependent changes in adductor muscle swimming physiology of the scallop Aequipecten opercularis J. Exp. Biol., August 1, 2008; 211(15): 2492 - 2501. [Abstract] [Full Text] [PDF]

				C. M. Stary, B. J. Walsh, A. E. Knapp, D. Brafman, and M. C. Hogan Elevation in heat shock protein 72 mRNA following contractions in isolated single skeletal muscle fibers Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R642 - R648. [Abstract] [Full Text] [PDF]

				S. T. Kinsey, K. M. Hardy, and B. R. Locke The long and winding road: influences of intracellular metabolite diffusion on cellular organization and metabolism in skeletal muscle J. Exp. Biol., October 15, 2007; 210(20): 3505 - 3512. [Abstract] [Full Text] [PDF]

				C. A. Kindig, B. Walsh, R. A. Howlett, C. M. Stary, and M. C. Hogan Relationship between intracellular PO2 recovery kinetics and fatigability in isolated single frog myocytes J Appl Physiol, June 1, 2005; 98(6): 2316 - 2319. [Abstract] [Full Text] [PDF]