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This Article Full Text Full Text (PDF) All Versions of this Article: 90/4/507    most recent expphysiol.2004.029801v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morán, M. Articles by Megías, A. Search for Related Content PubMed PubMed Citation Articles by Morán, M. Articles by Megías, A.

Department of Biochemistry and Molecular Biology I, Faculties of ¹ Biology² Chemistry, Complutense University, 28040 Madrid, Spain

Isolated Langendorff-perfused hearts from sedentary and prolonged (24 weeks) treadmill-trained rats were subjected to 30 min of normoxic perfusion either alone or followed by 20 min of global ischaemia, or by 20 min of global ischaemia and 15 min of normoxic reperfusion. Pre-ischaemic values of antioxidant enzyme activities and ecto-5'-nucleotidase activity were not different in sedentary and trained hearts but a 5-fold increase of 72-kDa heat shock protein (HSP72) levels was detected in trained myocardium. After ischaemia and reperfusion (I/R), metabolic recovery was better in trained than in sedentary hearts as indicated by higher ATP and creatine phosphate levels. However, antioxidant enzymatic activities, glutathione reductase, and total and mitochondrial superoxide dismutase decreased in trained rats after I/R, whereas they remained unchanged in the sedentary ones. Ecto-5'-nucleotidase activity was modified by I/R in sedentary as well as in trained hearts while HSP72 content did not change. Ecto-5'-nucleotidase activity and HSP72 content increased in parallel by the 30-min perfusion period. In conclusion, the cardioprotection induced by long-term training could be mediated by the exercise-induced increase in HSP72 levels and is not related to enhanced antioxidant systems or ecto-5'-NT activity.

(Received 4 January 2005; accepted after revision 21 February 2005; first published online 8 March 2005)
Corresponding author A. Megías: Departamento de Bioquimica y Biologia Molecular I, Facultad de Biología, Universidad Complutense, 28040 Madrid, España.  Email: amegias{at}bbm1.ucm.es

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