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This Article Full Text Full Text (PDF) All Versions of this Article: 93/5/701    most recent expphysiol.2008.041988v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Google Scholar Articles by Shan, Z. Articles by Raizada, M. K. PubMed PubMed Citation Articles by Shan, Z. Articles by Raizada, M. K. Related Collections Cardiovascular control

¹ Department of Physiology and Functional Genomics, University of Florida, College of Medicine, Gainesville, FL 32610, USA

(Pro)renin receptor (PRR), the newest member of the renin–angiotensin system (RAS), is turning out to be an important player in the regulation of the cardiovascular system. It plays a pivotal role in activation of the local RAS and stimulates signalling pathways involved in proliferative and hypertrophic mechanisms. However, the role of PRR in the brain remains unknown. Thus, our objective in this study was to determine whether a functional PRR is present in neurons within the brain. Neuronal co-cultures from the hypothalamus and brainstem areas of neonatal rat brain express PRR mRNA. Immunoreactivity for PRR was primarily localized on the neuronal cell soma and in discrete areas in the neurites. Treatment of neurons with renin, in the presence of 2 µM losartan, caused a time- and dose-dependent stimulation of phosphorylation of extracellular signal related kinase ERK1 (p44) and ERK2 (p42) isoforms of mitogen-activated protein kinase. Optimal stimulation of fourfold was observed within 2 min with 20 nM renin. Electrophysiological recordings showed that treatment of the neurons with renin, in the presence of 2 µM losartan, resulted in a steady and stable decrease in action potential frequency. A 46% decrease in action potential frequency was observed within 5 min of treatment and was attenuated by co-incubation with a PRR blocking peptide. These observations demonstrate that the PRR is present in neurons within the brain and that its activation by renin initiates the MAP kinase signalling pathway and inhibition of neuronal activity.

(Received 7 January 2008; accepted after revision 3 March 2008; first published online 7 March 2008)
Corresponding author M. K. Raizada: University of Florida, Department of Physiology, 1600 SW Archer Road, PO Box 100274, Gainesville, FL 32610, USA. Email: mraizada{at}phys.med.ufl.edu

This article has been cited by other articles:

				J. L. Grobe, D. Xu, and C. D. Sigmund An Intracellular Renin-Angiotensin System in Neurons: Fact, Hypothesis, or Fantasy Physiology, August 1, 2008; 23(4): 187 - 193. [Abstract] [Full Text] [PDF]

				M. K. Raizada and J. F. R. Paton Recent advances in the renin-angiotensin system: angiotensin-converting enzyme 2 and (pro)renin receptor Exp Physiol, May 1, 2008; 93(5): 517 - 518. [Full Text] [PDF]