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This Article Full Text Full Text (PDF) All Versions of this Article: 91/1/249    most recent expphysiol.2005.032482v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fujiwara, S. Articles by Nakahari, T. Search for Related Content PubMed PubMed Citation Articles by Fujiwara, S. Articles by Nakahari, T. Related Collections GI & Epithelial

¹ Department of Physiology² Department of Internal Medicine (Division II)³ Central Research Laboratory (Nakahari Project), Osaka Medical College, 2-7 Daigakucho, Takatsuki 569-8686, Japan

Ca²⁺-regulated exocytosis is enhanced by an autocrine mechanism via the PGE₂–cAMP pathway in antral mucous cells of guinea-pigs. The inhibition of the PGE₂–cAMP pathway by H-89 (an inhibitor of protein kinase A, PKA) or aspirin (ASA, an inhibitor of cyclo-oxygenase, COX) decreased the frequency of ACh-stimulated exocytotic events by 60%. Indomethacin (IDM, an inhibitor of COX), however, decreased the frequency of ACh-stimulated exocytotic events only by 30%. Moreover, IDM increased the frequency of ACh-stimulated exocytotic events by 50% in H-89-treated or ASA-treated cells. IDM inhibits the synthesis of Prostaglandin (PGG/H) and (15R)-15-hydroxy-5,8,11 cis-13-trans-eicosatetraenoic acid (15R-HPETE), while ASA inhibits only the synthesis of PGG/H. Thus, IDM may accumulate arachidonic acid (AA). AACOCF₃ or N-(p-amylcinnamoyl) anthranilic acid (ACA; both inhibitors of phospholipase A₂, PLA₂), which inhibits AA synthesis, decreased the frequency of ACh-stimulated exocytotic events by 60%. IDM, however, did not increase the frequency in AACOCF₃-treated cells. AA increased the frequency of ACh-stimulated exocytotic events in AACOCF₃- or ASA-treated cells, similar to IDM in ASA- and H-89-treated cells. Moreover, in the presence of AA, IDM did not increase the frequency of ACh-stimulated exocytotic events in ASA-treated cells. The PGE₂ release from antral mucosa indicates that inhibition of PLA₂ by ACA inhibits the AA accumulation in unstimulated and ACh-stimulated antral mucosa. The dose–response study of AA and IDM demonstrated that the concentration of intracellular AA accumulated by IDM is less than 100 nM. In conclusion, IDM modulates the ACh-stimulated exocytosis via AA accumulation in antral mucous cells.

(Received 6 October 2005; accepted after revision 1 November 2005; first published online 1 November 2005)
Corresponding author T. Nakahari: Department of Physiology, Osaka Medical College, 2-7 Daigakucho, Takatsuki 569-8686, Japan. Email: takan{at}art.osaka-med.ac.jp