Experimental Physiology Celebrating 100 years
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiology in Press

First published online on May 4, 2006.
Experimental Physiology (2006)
DOI: 10.1113/expphysiol.2006.033274
© The Physiological Society 2006
A more recent version of this article appeared on July 1, 2006
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
91/4/755    most recent
expphysiol.2006.033274v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Author home page(s):
Brian Lindegaard Pedersen
Henrik Arendrup
Niels Henry Secher
Bjørn Quistorff
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pedersen, B. L.
Right arrow Articles by Quistorff, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedersen, B. L.
Right arrow Articles by Quistorff, B.

Received January 20, 2006
Revised March 8, 2006
Accepted after revision April 28, 2006

Muscle [260]

31PMRS evaluated metabolism of perfused intercostal muscles

Brian Lindegaard Pedersen 1*, Henrik Arendrup 2, Niels Henry Secher 3, Bjørn Quistorff 4

1 IMBG-A, NMR-center
2 Department of Thoracic Surgery,Rigshospitalet, University of Copenhagen, Denmark.
3 Department of Anaesthesia, Rigshospitalet, University of Copenhagen, Denmark.
4 IMBG-A. NMR-center

* To whom correspondence should be addressed. E-mail: brlp{at}imbg.ku.dk.


  Abstract
Summary This study presents a perfused preparation for evaluation of metabolism in pig intercostal muscle in vitro. Preserved vessels and nerves to an intercostal segment including two adjacent ribs allowed for tissue perfusion and electrical stimulation with measurement of contraction force, oxygen consumption, and 31P-magnetic resonance spectroscopy (MRS). When perfused at rest with Krebs-Ringer buffer the preparation maintained physiological levels of PCr, Pi, ATP and pH at a stable oxygen consumption of ~ 0.5 µmoles min-1g-1 for more than 2 h. Tonic stimulation of the nerve caused anaerobic energy consumption as PCr and pH decreased and both variables recovered after the contraction with t1/2 values of ~ 7 min. Force increased to 0.040 N g-1 (range 0.031-0.103) and it gradually decreased by about 70% during the subsequent 5 min of stimulation. The calculated free ADP concentration increased from 7.4±2.1 at rest to 28±12 nmoles g-1 (mean ±SD) by the end of the stimulation. Thus anaerobic ATP turnover was 0 at rest, 6.1±2 µmoles min-1 g-1 during the first minute of stimulation and 3.5±0.5 during the two last minutes, corresponding to the drop in force. When the preparation was left unperfused anaerobic ATP turnover averaged 0.40±0.15 µmoles min-1 g-1 for the first 10 min. The preparation can also be applied to human intercostal muscles, as demonstrated for one biopsy. The results demonstrate a stable and functional in vitro preparation of intact perfused intercostal muscles in the pig. Key words: ATP; PCr; force; phosphorous magnetic resonance spectroscopy; oxygen consumption; intramuscular pH.

Key Words: Muscle, Nuclear magnetic resonance, Perfusion






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by the The Physiological Society.