Experimental Physiology Celebrating 100 years
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiology in Press

First published online on February 15, 2007.
Experimental Physiology (2007)
DOI: 10.1113/expphysiol.2006.036228
© The Physiological Society 2007
A more recent version of this article appeared on May 1, 2007
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
92/3/541    most recent
expphysiol.2006.036228v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alders, D. J.
Right arrow Articles by van Beek, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alders, D. J.
Right arrow Articles by van Beek, J. H.

Received November 15, 2006
Revised January 3, 2007
Accepted after revision February 9, 2007

Heart/Cardiac Muscle [240]

Regional sympathetic denervation affects the relation between local canine myocardial blood flow and oxygen consumption

David JC Alders 1, Richard N. Cornelussen 2, Frits W Prinzen 2, Patricia AC Specht 1, Mark I.M. Noble 3*, Angela J Drake-Holland 4, Frans JJ de Kanter 1, Johannes HGM van Beek 1

1 Free University of Amsterdam
2 University of Maastricht
3 University of Aberdeen
4 Robert Gordon University, Aberdeen

* To whom correspondence should be addressed. E-mail: mimnoble{at}mac.com.


  Abstract
Myocardial blood flow and oxygen consumption are heterogeneously distributed. Perfusion and myocardial oxygen consumption are closely correlated in the normal heart. It is unknown how this metabolism - perfusion relation is influenced by sympathetic denervation. We investigated this question in seven chloralose anesthetized dogs, 3 to 4 weeks after regional sympathetic denervation of left circumflex coronary artery area of supply of the left ventricle. Measurements were made of local myocardial blood flow (MBF, ml.min-1.gm dry wt-1), measured with microspheres, and myocardial oxygen consumption (MVO2, µmol.min-1.gm dry wt-1) in the same location, calculated from the 13C spectrum of tissue extracts after intra-coronary infusion of 3-13C-lactate. As both innervated and denervated regions are subject to the same arterial pressure, lower blood flow indicates higher resistance. Mean MBF was 5.56 (heterogeneity of 3.47) innervated, 7.48 (heterogeneity of 3.62) denervated (NS). Significant linear relations were found between MBF and MVO2 of individual samples within the innervated and denervated regions. The slopes of these relations were not significantly different, but the adjusted mean was significantly higher in the denervated regions (+1.92, an increase of 38% of the mean MBF at the pooled mean MVO2, p = 0.028, ANCOVAR). The ratio MBF/MVO2 (ml.µmol-1) was significantly higher, 0.296±0.167 in the denervated region compared with the innervated region, 0.216±0.126 p = 0.0182, Mann-Whitney test. These results indicate that sympathetic tone under chloralose anaesthesia imposes a moderate vasoconstrictive effect in the myocardium that is not detected by comparison of the mean blood flow or resistance.

Key Words: Heart, Magnetic resonance, Metabolism






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the The Physiological Society.