Received October 3, 2007
Revised November 6, 2007
Accepted after revision January 10, 2008
Antioxidants attenuate oxidative damage in skeletal muscle during mild ischemia
Andrew Judge 1*,
Joshua Selsby 2,
Stephen Dodd 1
1 University of Florida
2 University of Pennsylvania
* To whom correspondence should be addressed. E-mail: ajudge{at}hhp.ufl.edu.
 |
Abstract |
|---|
We have previously shown oxidative stress and edema within skeletal muscle after contractile-induced claudication, which is caused by both xanthine oxidase-derived oxidants and infiltrating neutrophils. The purpose of this study was to determine whether supplementation with antioxidant vitamins attenuates oxidative stress, neutrophil infiltration and edema, associated with an acute bout of contractile-induced claudication. Rats received either: vehicle, vitamin C, vitamin E, or vitamin C+E for 5 days prior to contractile-induced claudication. Force production was significantly reduced in the claudicant limbs of all groups compared to the control (sham) limb of control animals. Contractile-induced claudication caused a significant increase in protein oxidation, lipid peroxidation, neutrophil infiltration and edema compared to sham muscles. Supplementation with vitamins C, E, and C+E prevented the increases in each of these and there were no differences between groups. These findings suggest that, in an animal model of exercise claudication, neutrophil chemotaxis is caused by oxidizing species and that antioxidant supplementation can prevent oxidative damage, neutrophil infiltration, and edema following an acute bout of contractile-induced claudication.
Key Words:
Antioxidant, Ischaemia, Skeletal muscle
